Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Aged Tendon Stem/Progenitor Cells Are Less Competent to Form 3D Tendon Organoids Due to Cell Autonomous and Matrix Production Deficits

doi: 10.3389/fbioe.2020.00406

Figure Lengend Snippet: Collagen I protein production and gene expression profiling by Real Time PCR. (A) Representative collagen I immunofluorescent images of Y-TSPC and A-TSPC sheets at day 14. (B) Quantitative analysis of collagen I by ELISA. For (A,B) Y-TSPC n = 3; A-TSPC n = 6. Normalization to DNA. Quantitative PCR analysis for tendon-transcription factors (C) , collagen genes (D) , cross-linker genes (E) , tendon-related ECM genes (F) other lineage genes (G) . Gene expression was calculated as fold change to Y-TSPC group. GAPDH was used as reference gene in each group. Non-detectable (Ct over 38 genes were not included in the graphs. For (C–G) Y-TSPC n = 3; A-TSPC n = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Next, collagen I Elisa kit (Chondrex, Redmond, WA, United States) was used according to the manufacturer’s instruction.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

Journal: Journal of Cardiothoracic Surgery

Article Title: GPER1-mediated suppression of acute cellular rejection in murine cervical heart transplantation by estradiol

doi: 10.1186/s13019-025-03432-8

Figure Lengend Snippet: Coronary Intimal Hyperplasia Analysis via Elastic Fiber Staining. A : Syngeneic group: No evident intimal hyperplasia was observed (931.95 ± 145.86 μm²). B : Allogenic control group: There was a significant reduction in intimal thickening compared to the allogenic control group (12783.69 ± 1793.18 μm²). C : E2 group: Significant reduction in intimal thickening compared to the allogenic control group (2531.00 ± 286.83 μm²). D : E2 + G15 group: Intimal hyperplasia was more pronounced compared to the E2 group (9178.53 ± 3393.69 μm²). Scale bar: 50 μm; magnification: ×200

Article Snippet: Hematoxylin and eosin (H&E) staining kits and elastic fiber staining kits (Gomori aldehyde magenta) were obtained from Beijing Solarbio Science & Technology Co., Ltd., and primers and GAPDH were sourced from Servicebio, please reference Table for the primers.

Techniques: Staining, Control

Journal: The FASEB Journal

Article Title: Response gene to complement 32 suppresses adipose tissue thermogenic genes through inhibiting β3-adrenergic receptor/mTORC1 signaling

doi: 10.1096/fj.201701508R

Figure Lengend Snippet: Rgc32−/− promoted CL 316243-induced iWAT thermogenic gene expression and energy expenditure. WT and Rgc32−/− mice were injected with vehicle or CL 316243 (1 mg/kg body weight, i.p.) for 5 d (n = 6). A, B) Representative images of H&E (A) and immunohistochemistry (B) staining for UCP-1 of iWAT as indicated. C, D) Protein expression of PGC-1α and UCP-1 in iWAT was detected by Western blot analysis. *P < 0.05, **P < 0.01 vs. WT treated with vehicle groups; ##P < 0.01 vs. WT treated with CL 316243 groups. E) Energy expenditure of the mice injected with CL 316243 was measured by indirect calorimetry in both light and dark cycles. **P < 0.01 vs. WT Light groups, ##P < 0.01 vs. WT Dark groups. Scale bars, 200 μm.

Article Snippet: Hematoxylin and eosin (H&E) staining was performed with an H&E Stain Kit (KTHNEPT; American Mastertech Scientific, Lodi, CA, USA), according to the manufacturer’s instructions.

Techniques: Expressing, Injection, Immunohistochemistry, Staining, Western Blot

Journal: The FASEB Journal

Article Title: Response gene to complement 32 suppresses adipose tissue thermogenic genes through inhibiting β3-adrenergic receptor/mTORC1 signaling

doi: 10.1096/fj.201701508R

Figure Lengend Snippet: β3-AR signaling is necessary for Rgc32−/−-induced iWAT thermogenic gene expression. A, B) SVF cells of iWAT was isolated from age-matched male WT and Rgc32−/− mice, and adipocyte differentiation was induced along with vehicle or SR 59230A (1 μM) treatment. PGC-1α and UCP-1 expression was detected by Western blot analysis (n = 3). C, D) WT and Rgc32−/− mice were injected with CL 316243 (1 mg/kg body weight, i.p.) along with vehicle or SR 59230A (5 mg/kg body weight, s.c.) for 5 d (n = 6). Shown are the representative images of H&E (C) and immunohistochemistry (D) staining for UCP-1 of iWAT. E) Quantitative analysis of UCP-1 staining density as shown in D. F, G) PGC-1α and UCP-1 expression in iWAT was detected by Western blot analysis. *P < 0.05, **P < 0.01 vs. WT treated with vehicle groups, #P > 0.05 vs. WT treated with SR 59230A groups. Scale bars, 200 μm.

Article Snippet: Hematoxylin and eosin (H&E) staining was performed with an H&E Stain Kit (KTHNEPT; American Mastertech Scientific, Lodi, CA, USA), according to the manufacturer’s instructions.

Techniques: Expressing, Isolation, Western Blot, Injection, Immunohistochemistry, Staining

Journal: The FASEB Journal

Article Title: Response gene to complement 32 suppresses adipose tissue thermogenic genes through inhibiting β3-adrenergic receptor/mTORC1 signaling

doi: 10.1096/fj.201701508R

Figure Lengend Snippet: Rgc32−/− promoted iWAT thermogenic gene expression through mTORC1 activation. A, B) SVF of iWAT was isolated from age-matched male WT and Rgc32−/− mice, and adipocyte differentiation was induced. p-S6K1 was detected by Western blot analysis (n = 3). C, D) p-S6K1 in iWAT from age-matched male WT and Rgc32−/− mice was detected by Western blot analysis (n = 6). **P < 0.01 vs. WT groups. E, F) SVF of iWAT was isolated from age-matched male WT and Rgc32−/− mice, and adipocyte differentiation was induced along with vehicle or rapamycin (100 nM) treatment. p-S6K1 and UCP-1 levels was detected by Western blot analysis (n = 3). G, H) WT and Rgc32−/− mice were injected with CL 316243 (1 mg/kg body weight, i.p.) along with vehicle or rapamycin (2.5 mg/kg body weight, i.p.) for 5 d (n = 6). Representative images of H&E (G) and immunohistochemistry (H) staining for UCP-1 of iWAT were shown as indicated. I) Quantitative analysis of UCP-1 staining density as shown in H. J, K) PGC-1α and UCP-1 expression in iWAT was detected by Western blot analysis. *P < 0.05, **P < 0.01 vs. WT treated with vehicle groups, #P > 0.05 vs. WT treated with rapamycin groups. Scale bars, 200 μm.

Article Snippet: Hematoxylin and eosin (H&E) staining was performed with an H&E Stain Kit (KTHNEPT; American Mastertech Scientific, Lodi, CA, USA), according to the manufacturer’s instructions.

Techniques: Expressing, Activation Assay, Isolation, Western Blot, Injection, Immunohistochemistry, Staining